DSL-6A/C1 大鼠胰腺癌細胞

產(chǎn)品型號：CRL-2132
廠商性質：生產(chǎn)廠家
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細胞原代牛、狗、雞、鴨、魚原代細胞羊原代細胞豬原代細胞兔原代細胞小鼠原代細胞大鼠原代細胞人原代細胞培養(yǎng)基原代細胞凍存液 ATCC 人乳腺癌豬正常細胞豬睪丸細胞系人膠質瘤細胞人成骨細胞人永生化肝細胞人肝癌細胞人卵巢癌細胞人結直腸腺癌人經(jīng)典小細胞肺癌細胞人小細胞肺癌細胞人結直腸腺癌細胞人鼻腔上皮細胞中國倉鼠卵巢懸浮細胞嗜性逆轉錄病毒包裝穩(wěn)定細胞株小鼠神經(jīng)細胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉錄酶病毒包裝細胞鴨子胚胎成纖維細胞豬腎細胞系昆蟲細胞貓腎細胞恒河猴胚腎細胞倉鼠卵巢細胞負鼠腎細胞倉鼠肺細胞果蠅胚胎細胞家貓肺成纖維樣細胞雞胚細胞豚鼠心肌來源細胞豚鼠肺成纖維樣細胞豚鼠皮膚成纖維樣細胞狗腎細胞非洲綠猴胚胎腎上皮細胞猴胚胎腎上皮細胞長臂猿淋巴瘤新生牛眼囊成纖維細胞黑化大家鼠肺成纖維樣細胞俄勒岡地鼠細胞家兔皮膚成纖維樣細胞昆蟲卵巢細胞人胸膜瘤細胞非洲綠猴腎細胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細胞黃胸鼠肺成纖維樣細胞白鰱尾鰭細胞蝙蝠肺細胞草魚腎細胞系大黃魚腎細胞猴腎細胞系犬腎細胞系人結腸直腸腺癌豬肺泡巨噬細胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導因子人乳腺癌細胞結腸癌細胞成纖維細胞人胚腎細胞動物卵巢細胞動物胚胎細胞動物肺細胞肋骨來源細胞成纖維樣細胞動物上皮細胞動物肝臟細胞可誘導表達穩(wěn)定細胞株動物氣管細胞動物腎細胞動物巨噬細胞人正常細胞人腫瘤細胞、癌細胞小鼠正常細胞小鼠腫瘤細胞、癌細胞大鼠正常細胞大鼠腫瘤細胞其它動物細胞查看全部產(chǎn)品

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CRL-2132 DSL-6A/C1 大鼠胰腺癌細胞

ATCC^® Number:

CRL-2132™

Price:

$438.00

Designations:	DSL-6A/C1
Depositors:	OS Pettengill, D Longnecker
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Rattus norvegicus （rat）
Morphology:	epithelial
Source:	Organ: pancreas Strain: Lewis Disease: pancreatic carcinoma, azaserine induced
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2132
Restrictions:	Note: These cells are distributed subject to the following: 1.） This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.） Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. （603） 646-3675. 3.） In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
Tumorigenic:	Yes
Gender:	male
Comments:	DSL-6A/C1 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma. The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat （DSL-101-79） that was given azaserine intraperitoneally. The cultured DSL-6A/C1 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture. The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting. The DSL-6A/C1 cell line expresses the ductal marker cystic fibrosis transmembrane regulator （CFTR）.
Propagation:	ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing:	*Subction Ratio:** A subction ratio of 1:3 to 1:4 is recommended Medium Renewal:* Twice per week Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline （D-PBS） or 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C.
Preservation:	Culture medium, 95%; DMSO, 5%
References:	23188: Durfee T, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am. J. Pathol. 143: 292-303, 1993. PubMed: 8391218

ATCC® Number: CRL-2132™ Price: $438.00

Designations: DSL-6A/C1

Depositors: OS Pettengill, D Longnecker

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus （rat）

Morphology: epithelial

Source: Organ: pancreas

Strain: Lewis

Disease: pancreatic carcinoma, azaserine induced

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Note: These cells are distributed subject to the following: 1.） This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.） Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. （603） 646-3675. 3.） In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

Tumorigenic: Yes

Gender: male

Comments: DSL-6A/C1 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.

The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat （DSL-101-79） that was given azaserine intraperitoneally.

The cultured DSL-6A/C1 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.

The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting.

The DSL-6A/C1 cell line expresses the ductal marker cystic fibrosis transmembrane regulator （CFTR）.

Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%

Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline （D-PBS） or 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

5.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Preservation: Culture medium, 95%; DMSO, 5%

References: 23188: Durfee T, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am. J. Pathol. 143: 292-303, 1993. PubMed: 8391218

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DSL-6A/C1 大鼠胰腺癌細胞

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